| Title | [The study on the relationship between expression of B7-H1 on HBV transgenic mice and immune tolerance to HBV.] | | Author(s) | Wang ZY, He JJ, Geng L, Zhou L, Xie HY, Wu J, Zheng SS | | Institution | Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, Key Laboratory of Organ Transplantation, Zhejiang Province, Hangzhou 310003,China. | | Source | Zhonghua Gan Zang Bing Za Zhi 2009 Oct; 17(10):750-3. | | Abstract | OBJECTIVE: To investigate whether there is an association between the expression of B7-H1 in HBV transgenic mice and the immune tolerance to HBV.
METHODS: T cells stimulatory capacity of DC was analyzed using mixed lymphocyte reaction. Expression of MHC-II, CD80, CD86, B7-H1 on DC was detected by Flow Cytometry. IL-2, IFNgamma, IL-10 production of T cells were determined by using ELISA. B7-H1 mRNA and protein expression in liver tissue were detected by RT-PCR and Western blotting respectively.
RESULTS: The ability of DC cells from HBV transgenic mice to stimulate T cell proliferation was significantly impaired compared with DC cells from control mice (t = 16.674, 19.674, 21.712, P less than 0.01). Expression of MHC-II, CD80 on DC was markedly decreased in transgenic mice (t = 7.910, 6.413, P less than 0.05). Meanwhile, the expression of CD86 and B7-H1 on DC cells in HBV transgenic mice were not significantly different from that in control mice. The levels of IL-2, IFNgamma, IL-10 in supernantant of T cells was significantly lower compared with controls (t = 18.712, 18.712 and 11.683, P less than 0.05). There was no significant difference in B7-H1 expression at mRNA and protein levels in liver tissue compared with controls.
CONCLUSIONS: Functional defeact of DC, partly due to decreased expression of MHC-II, CD80, but not related to B7-H1 expression, is the cause for immune tolerance to HBV in HBV transgenic mice. DOI: 10.3760/cma.j.issn.1007-3418.2009.10.008. | | Language | chi | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 19874690 |
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